The culture dishes are kept in a modular chamber which holds the gaseous environment very strictly and these are kept in turn in a CO2 incubator. Our IVF lab utilizes TRIGAS (O2, CO2, N2 mixture) incubator instead of CO2 alone , since the presence of 5 % O2 improves the culture status and integrity of embryos.
After 48 hours the cell division shows 4 cells inside the embryos and after 72 hours they show 8 cell divisions. Depending on the number of 8 cell stage the viability of the culture system is assessed. The success rates are also decided according to this.